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primary antibodies against human abca12  (GeneTex)
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GeneTex primary antibodies against human abca12
Different barrier functions and gene expression profiles of permeability barrier components characterize healthy GP, AGR, and SGR skin regions. ( a ) Transepidermal water loss levels in healthy GP, AGR, and SGR skin areas. Measurements were carried out with Tewameter TM300 (Courage and Khazaka, Cologne, Germany) on the flexural forearm, axilla, and forehead of healthy individuals ( n = 30). Gene expression of the components of ( b ) cornified envelope formation, ( c ) corneocyte desquamation, ( d ) intercellular lipid lamellae, ( e ) desmosome organization, and ( f ) tight junction formation in healthy GP, AGR, and SGR skin regions were examined by qRT-PCR. The graphs show the mean ± 95% confidence interval of measured mRNA transcript levels (* p < 0.05, ** p < 0.01, *** p < 0.001, determined by one-way ANOVA followed by Sidac post hoc test). Abbreviations: <t>ABCA12,</t> ABC transporter 12B; AGR, apocrine gland-rich; CDH, cadherin; CDSN, corneodesmosin; CLDN, claudin; DSC, desmocollin; DSG, desmoglein; GP, gland-poor; KLK, kallikrein-related peptidase; KRT, keratin; LCE, late cornified envelope; OCLN, occludin; PKP, plakophilin; SGR, sebaceous gland-rich; SPRR, small proline-rich protein; TEWL, transepidermal water loss; TGM, transglutaminase.
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Different barrier functions and gene expression profiles of permeability barrier components characterize healthy GP, AGR, and SGR skin regions. ( a ) Transepidermal water loss levels in healthy GP, AGR, and SGR skin areas. Measurements were carried out with Tewameter TM300 (Courage and Khazaka, Cologne, Germany) on the flexural forearm, axilla, and forehead of healthy individuals ( n = 30). Gene expression of the components of ( b ) cornified envelope formation, ( c ) corneocyte desquamation, ( d ) intercellular lipid lamellae, ( e ) desmosome organization, and ( f ) tight junction formation in healthy GP, AGR, and SGR skin regions were examined by qRT-PCR. The graphs show the mean ± 95% confidence interval of measured mRNA transcript levels (* p < 0.05, ** p < 0.01, *** p < 0.001, determined by one-way ANOVA followed by Sidac post hoc test). Abbreviations: ABCA12, ABC transporter 12B; AGR, apocrine gland-rich; CDH, cadherin; CDSN, corneodesmosin; CLDN, claudin; DSC, desmocollin; DSG, desmoglein; GP, gland-poor; KLK, kallikrein-related peptidase; KRT, keratin; LCE, late cornified envelope; OCLN, occludin; PKP, plakophilin; SGR, sebaceous gland-rich; SPRR, small proline-rich protein; TEWL, transepidermal water loss; TGM, transglutaminase.

Journal: International Journal of Molecular Sciences

Article Title: Regional Differences in the Permeability Barrier of the Skin—Implications in Acantholytic Skin Diseases

doi: 10.3390/ijms221910428

Figure Lengend Snippet: Different barrier functions and gene expression profiles of permeability barrier components characterize healthy GP, AGR, and SGR skin regions. ( a ) Transepidermal water loss levels in healthy GP, AGR, and SGR skin areas. Measurements were carried out with Tewameter TM300 (Courage and Khazaka, Cologne, Germany) on the flexural forearm, axilla, and forehead of healthy individuals ( n = 30). Gene expression of the components of ( b ) cornified envelope formation, ( c ) corneocyte desquamation, ( d ) intercellular lipid lamellae, ( e ) desmosome organization, and ( f ) tight junction formation in healthy GP, AGR, and SGR skin regions were examined by qRT-PCR. The graphs show the mean ± 95% confidence interval of measured mRNA transcript levels (* p < 0.05, ** p < 0.01, *** p < 0.001, determined by one-way ANOVA followed by Sidac post hoc test). Abbreviations: ABCA12, ABC transporter 12B; AGR, apocrine gland-rich; CDH, cadherin; CDSN, corneodesmosin; CLDN, claudin; DSC, desmocollin; DSG, desmoglein; GP, gland-poor; KLK, kallikrein-related peptidase; KRT, keratin; LCE, late cornified envelope; OCLN, occludin; PKP, plakophilin; SGR, sebaceous gland-rich; SPRR, small proline-rich protein; TEWL, transepidermal water loss; TGM, transglutaminase.

Article Snippet: Sections were stained with primary antibodies against human ABCA12 (rabbit polyclonal IgG, GTX51202, GeneTex), human CDSN (rabbit polyclonal IgG, HPA054184, Sigma-Aldrich, St. Louis, MO, USA), human CLDN1 (rabbit polyclonal IgG, ab15098, Abcam, Cambridge, UK), human DSG1 (rabbit polyclonal IgG, NBP1-84567, Novus Biologicals, Centennial, CO, USA), human KLK5 (rabbit polyclonal IgG, ab7283, Abcam), human KRT1 (rabbit monoclonal IgG, ab185628, Abcam), human OCLN (rabbit monoclonal IgG, ab216327, Abcam), and human TGM5 (rabbit polyclonal IgG, ab133786, Abcam).

Techniques: Gene Expression, Permeability, Quantitative RT-PCR

Prominently weakened cell junction components characterize SGR and AGR skin regions compared with GP areas under steady-state. Representative images for immunostaining and quantification of epidermal levels of KRT1, TGM5, CDSN, DSG1, CLDN1, OCLN, ABCA12, KLK5, and KLK7 in healthy GP, AGR, and SGR skin sections. Bar = 100 μm. Means ± 95% confidence intervals for protein levels are shown. (* p < 0.05, ** p < 0.01, *** p < 0.001, determined by one-way ANOVA followed by Sidac post hoc test) Abbreviations: ABCA12, ABC transporter 12B; AGR, apocrine gland-rich; CDSN, corneodesmosin; CLDN, claudin; DSG1, desmoglein 1; GP, gland-poor; KLK, kallikrein-related peptidase; KRT, keratin; OCLN, occludin; SGR, sebaceous gland-rich; TGM, transglutaminase.

Journal: International Journal of Molecular Sciences

Article Title: Regional Differences in the Permeability Barrier of the Skin—Implications in Acantholytic Skin Diseases

doi: 10.3390/ijms221910428

Figure Lengend Snippet: Prominently weakened cell junction components characterize SGR and AGR skin regions compared with GP areas under steady-state. Representative images for immunostaining and quantification of epidermal levels of KRT1, TGM5, CDSN, DSG1, CLDN1, OCLN, ABCA12, KLK5, and KLK7 in healthy GP, AGR, and SGR skin sections. Bar = 100 μm. Means ± 95% confidence intervals for protein levels are shown. (* p < 0.05, ** p < 0.01, *** p < 0.001, determined by one-way ANOVA followed by Sidac post hoc test) Abbreviations: ABCA12, ABC transporter 12B; AGR, apocrine gland-rich; CDSN, corneodesmosin; CLDN, claudin; DSG1, desmoglein 1; GP, gland-poor; KLK, kallikrein-related peptidase; KRT, keratin; OCLN, occludin; SGR, sebaceous gland-rich; TGM, transglutaminase.

Article Snippet: Sections were stained with primary antibodies against human ABCA12 (rabbit polyclonal IgG, GTX51202, GeneTex), human CDSN (rabbit polyclonal IgG, HPA054184, Sigma-Aldrich, St. Louis, MO, USA), human CLDN1 (rabbit polyclonal IgG, ab15098, Abcam, Cambridge, UK), human DSG1 (rabbit polyclonal IgG, NBP1-84567, Novus Biologicals, Centennial, CO, USA), human KLK5 (rabbit polyclonal IgG, ab7283, Abcam), human KRT1 (rabbit monoclonal IgG, ab185628, Abcam), human OCLN (rabbit monoclonal IgG, ab216327, Abcam), and human TGM5 (rabbit polyclonal IgG, ab133786, Abcam).

Techniques: Immunostaining